Oil-degrading bacterial consortium from Gulf of Mexico designed by a factorial method, reveals stable population dynamics
نویسندگان
چکیده
We describe an assembled marine bacterial consortium designed for bioremediation of oil-contaminated seawater, based on a statistical method using Plackett-Burman (PB) experimental approach. The final consists four bacteria isolated from the Gulf Mexico, genera: Pseudomonas, Halopseudomonas, Paenarthrobacter , and Alcanivorax . Individually, oil removal by these microorganisms was evaluated gravimetry, reaching 39% at maximum after 75 days, whereas in it ~62%. also measured biodegradation levels Gas Chromatography/Mass Spectrometry (GC-MS) observing 12 polyaromatic hydrocarbons (PAHs) degradation analyzed n -alkanes with preference specific chain length. Consortium population analysis V3-V4 region 16S rRNA showed stable community, suggesting that metabolic load distributed among dynamic interactions were achieved. In this work, we show use factorial synthetic design offers possibility improving efficiency populations.
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ژورنال
عنوان ژورنال: Frontiers in Marine Science
سال: 2022
ISSN: ['2296-7745']
DOI: https://doi.org/10.3389/fmars.2022.962071